Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.| Product Name | EIF4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4B, EIF-4B, PRO1843 |
| Product description | IF4B contains 1 RRM (RNA recognition motif) domain and is required for the binding of mRNA to ribosomes. Functions of EIF4B are in close association with EEF4-F and EIF4-A. It binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP and promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.1) Suzuki, Y., (2004) Genome Res. 14 (9), 1711-1718. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| EIF4B antibody can be used for detection of EIF4B by ELISA at 1:62500. EIF4B antibody can be used for detection of EIF4B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF4B |
|---|---|
| Alternative Names | EIF4B EIF-4B PRO1843 |
| Molecular Weight(MW) | 67 kDa |
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | EIF4B antibody can be used for detection of EIF4B by ELISA at 1:62500. EIF4B antibody can be used for detection of EIF4B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF4B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
