Application
Western blot analysis of lysed extracts from HeLa, HepG2 and 293 cells using eIF4E (Ab-209).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Ab-209). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using eIF4E (Ab-209).| Product Name | eIF4E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, A4, AD1, mRNA cap-binding protein, eIF-4F 25 kDa subunit |
| Product description | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.1) Li BD, et al. (1998) Ann Surg; 227 (5): 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18): 7520De Gregorio E, et al. (2001) RNA; 7 (1): 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15): 4448-4461Ohlmann T, et al. (1996) EMBO J; 15 (6): 1371-1382 |
| Immunogen | eIF4E (Ab-209) antibody was raised against a peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4E |
|---|---|
| Alternative Names | CBP EIF4F AUTS19 EIF4E1 EIF4EL1 A4 AD1 mRNA cap-binding protein eIF-4F 25 kDa subunit |
| Molecular Weight(MW) | 25 kDa |
| Tissue Specificity | This antibody detects endogenous level of total elF4E protein. |
| Entrez Gene | 1977 |
|---|---|
| Protein Accession | NP_001124150.1 |
Application
Western blot analysis of lysed extracts from HeLa, HepG2 and 293 cells using eIF4E (Ab-209).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Ab-209). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using eIF4E (Ab-209).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
