Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209).| Product Name | eIF4E (phospho Ser209) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, BCL2L3, mRNA cap-binding protein, eIF-4F 25 kDa subunit |
| Product description | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.1) Li BD, et al. (1998) Ann Surg; 227 (5): 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18): 7520De Gregorio E, et al. (2001) RNA; 7 (1): 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15): 4448-4461 |
| Immunogen | eIF4E (Phospho-Ser209) antibody was raised against a peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E. |
| Modification | p-Ser209 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4E |
|---|---|
| Alternative Names | CBP EIF4F AUTS19 EIF4E1 EIF4EL1 BCL2L3 mRNA cap-binding protein eIF-4F 25 kDa subunit |
| Molecular Weight(MW) | 25 kDa |
| Tissue Specificity | This antibody detects endogenous level of eIF4E only when phosphorylated at serine 209. |
| Entrez Gene | 1977 |
|---|---|
| Protein Accession | NP_001124150.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
