Application
Western blot analysis of lysed extracts from HepG2 and HT29 cells using eIF4G (Ab-1232).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (Ab-1232). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using eIF4G (Ab-1232).| Product Name | eIF4G Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, GAPD, CDABP0047, OK/SW-cl.12, |
| Product description | eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis1) De Gregorio, E. et al. (1998) RNA 4, 828-836. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382. Borman, A. M. and Kean, K. M. (1997) Virology 237, 129-136. Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42. |
| Immunogen | eIF4G (Ab-1232) antibody was raised against a peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4G1 |
|---|---|
| Alternative Names | P220 EIF4F EIF4G EIF4GI PARK18 EIF-4G1 GAPD CDABP0047 OK/SW-cl.12 |
| Molecular Weight(MW) | 220 kDa |
| Tissue Specificity | This antibody detects endogenous level of total eIF4G protein. |
| Entrez Gene | 1981 |
|---|---|
| Protein Accession | NP_004944.2 |
Application
Western blot analysis of lysed extracts from HepG2 and HT29 cells using eIF4G (Ab-1232).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (Ab-1232). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using eIF4G (Ab-1232).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
