Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.Application
Antibody used in WB on Human Hela at 1 ug/ml.| Product Name | EIF5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF5, EIF-5A, EIF-5 |
| Product description | EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EIF5 antibody can be used for detection of EIF5 by ELISA at 1:312500. EIF5 antibody can be used for detection of EIF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF5 |
|---|---|
| Alternative Names | EIF5 EIF-5A EIF-5 |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in WB on Human Hela at 1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | EIF5 antibody can be used for detection of EIF5 by ELISA at 1:312500. EIF5 antibody can be used for detection of EIF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
