Application
Antibody used in WB on Human Thymus at 2.5 ug/ml.| Product Name | ELF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ELF3, ERT, ESX, EPR-1, ESE-1 |
| Product description | ELF3 is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family. ELF3 contains two putative DNA binding domains: an ETS domain, which is unique in that the 5' half shows relatively weak homology to known ets factors, and an A/T hook domain, found in HMG proteins and various other nuclear factors. In contrast to any known ets factors, ELF3 is expressed exclusively in epithelial cells. ELF3 is expected to be a critical regulator of epithelial cell differentiation. ETS factor ELF3 is a transcriptional regulator of angiopoietin-1 gene regulation in the setting of inflammation. ELF3 overexpressed early during human breast tumorigenesis. ELF3 expression confers a transformed and in vitro metastatic phenotype to otherwise normal MCF-12A cells. ELF3 mediates the expression of TGF-beta RII, and the transcriptional inhibition of ets-related transcription factor could be a one of the mechanisms of colonic carcinogenesis. ELF3 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP.1) Wang, H., (2004) J. Biol. Chem. 279 (24), 25241-25250. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ELF3 antibody can be used for detection of ELF3 by ELISA at 1:312500. ELF3 antibody can be used for detection of ELF3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ELF3 |
|---|---|
| Alternative Names | ELF3 ERT ESX EPR-1 ESE-1 |
| Molecular Weight(MW) | 41 kDa |
Application
Antibody used in WB on Human Thymus at 2.5 ug/ml.| Positive Control | 1) Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate |
|---|---|
| Application Notes | ELF3 antibody can be used for detection of ELF3 by ELISA at 1:312500. ELF3 antibody can be used for detection of ELF3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ELF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
