Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Ab-389). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Elk1 (Ab-389).Application
Western blot analysis of lysed extracts from 293 and HT29 cells using Elk1 (Ab-389).| Product Name | Elk1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VIL2, ELK1, ETS-domain protein Elk-1 |
| Product description | Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene1) Janknecht R, et al. (1993) EMBO J. 12 (13): 5097-5104. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964. |
| Immunogen | Elk1 (Ab-389) antibody was raised against a peptide sequence around aa.387~391 (P-R-S-P-A) derived from Human Elk1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ELK1 |
|---|---|
| Alternative Names | VIL2 ELK1 ETS-domain protein Elk-1 |
| Molecular Weight(MW) | 62 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Elk1 protein. |
| Entrez Gene | 2002 |
|---|---|
| Protein Accession | NP_001107595.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Ab-389). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Elk1 (Ab-389).
Application
Western blot analysis of lysed extracts from 293 and HT29 cells using Elk1 (Ab-389).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
