Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.| Product Name | ELK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ELK1, |
| Product description | ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade.This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade.1) Hsieh, Y.H., et al., (2006) Biochem. Biophys. Res. Commun. 339 (1), 217-225. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELK1 antibody can be used for detection of ELK1 by ELISA at 1:312500. ELK1 antibody can be used for detection of ELK1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ELK1 |
|---|---|
| Alternative Names | ELK1 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ELK1 antibody can be used for detection of ELK1 by ELISA at 1:312500. ELK1 antibody can be used for detection of ELK1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ELK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
