Application
Western blot analysis of ELK1 using rabbit polyclonal ELK1 Antibody (S383) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELK1 gene (Lane 2).| Product Name | ELK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ETS domain-containing protein Elk-1, ELK1 |
| Product description | ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein is a nuclear target for the ras-raf-MAPK signaling cascade.1) Salinas, S., et al., J. Cell Biol. 165(6):767-773 (2004). |
| Immunogen | This ELK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 amino acids from human ELK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ELK1 |
|---|---|
| Alternative Names | ETS domain-containing protein Elk-1 ELK1 |
| Molecular Weight(MW) | 45 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of ELK1 using rabbit polyclonal ELK1 Antibody (S383) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELK1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
