Application
Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ELP2 antibody in NCI-H460 cell line lysates (35ug/lane)Application
Fluorescent confocal image of SY5Y cells stained with ELP2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ELP2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33| Product Name | ELP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Elongator complex protein 2, ELP2, SHINC-2, STAT3-interacting protein 1, StIP1, ELP2, STATIP1 |
| Product description | ELP2 regulates the ligand-dependent activation of STAT3. The protein acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.1) References for protein: |
| Immunogen | This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the C-terminal region of human ELP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ELP2 |
|---|---|
| Alternative Names | Elongator complex protein 2 ELP2 SHINC-2 STAT3-interacting protein 1 StIP1 ELP2 STATIP1 |
| Molecular Weight(MW) | 93 kDa |
Application
Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ELP2 antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Fluorescent confocal image of SY5Y cells stained with ELP2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ELP2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33| Application Notes | For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
