Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | EN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EN1, |
| Product description | Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Atit, R., (2006) Dev. Biol. 296 (1), 164-176. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EN1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EN1 antibody can be used for detection of EN1 by ELISA at 1:312500. EN1 antibody can be used for detection of EN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EN1 |
|---|---|
| Alternative Names | EN1 |
| Molecular Weight(MW) | 40 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | EN1 antibody can be used for detection of EN1 by ELISA at 1:312500. EN1 antibody can be used for detection of EN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
