Application
Antibody used in WB on Human cerebellum at 0.05 ug/ml.Application
Antibody used in IHC on Human Kidney.Application
Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.| Product Name | EN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EN2, AUTS1, AUTS10, |
| Product description | Homeobox-containing genes are thought to have a role in controlling development. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Wang, L., Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (4), 434-438. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EN2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EN2 antibody can be used for detection of EN2 by ELISA at 1:62500. EN2 antibody can be used for detection of EN2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EN2 |
|---|---|
| Alternative Names | EN2 AUTS1 AUTS10 |
| Molecular Weight(MW) | 34 kDa |
Application
Antibody used in WB on Human cerebellum at 0.05 ug/ml.
Application
Antibody used in IHC on Human Kidney.
Application
Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | EN2 antibody can be used for detection of EN2 by ELISA at 1:62500. EN2 antibody can be used for detection of EN2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EN2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
