Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml–2.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml–2.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hENA-78 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human ENA-78 (XP-5123Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.| Product Name | ENA-78 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCYB5, ENA-78, ENA78, SCYB5, C-X-C motif chemokine 5, Epithelial-derived neutrophil-activating protein 78, |
| Product description | ENA-78 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. ENA-78 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hENA 78 (human Epithelial Neutrophil Activating Peptide-78). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: To detect hENA-78 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hENA-78. Sandwich: To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human ENA-78 (XP-5123Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL5 |
|---|---|
| Alternative Names | SCYB5 ENA-78 ENA78 SCYB5 C-X-C motif chemokine 5 Epithelial-derived neutrophil-activating protein 78 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml–2.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml–2.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hENA-78 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human ENA-78 (XP-5123Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.| Application Notes | ELISA: To detect hENA-78 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hENA-78. Sandwich: To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human ENA-78 (XP-5123Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | ENA-78 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
