Application
Antibody used in WB on Human Jurkat 0.25 ug/ml.Application
Antibody used in IHC.| Product Name | ENO3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENO3, MSE, GSD13 |
| Product description | ENO3 is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.1) Li, T.B., (2004) Acta Biochim. Biophys. Sin. (Shanghai) 36 (6), 412-418. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ENO3 antibody can be used for detection of ENO3 by ELISA at 1:12500. ENO3 antibody can be used for detection of ENO3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENO3 |
|---|---|
| Alternative Names | ENO3 MSE GSD13 |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Human Jurkat 0.25 ug/ml.
Application
Antibody used in IHC.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ENO3 antibody can be used for detection of ENO3 by ELISA at 1:12500. ENO3 antibody can be used for detection of ENO3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ENO3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
