Application
Immunofluorescence staining of methanol-fixed HeLa cells using eNOS (Ab-1177).Application
Western blot analysis of lysed extracts from Rat heart, Mouse heart and Rat kidney tissue using eNOS (Ab-1177).| Product Name | eNOS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | eNOS, ECNOS, Constitutive NOS, EC-NOS, NOS3, NOSIII |
| Product description | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.1) Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M. B. et al. (2001) J. Biol. Chem. 276, 16587-16591. Thomas, S. R. et al. (2002) J. Biol. Chem. 277, 6017-6024. |
| Immunogen | eNOS (Ab-1177) antibody was raised against a peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOS3 |
|---|---|
| Alternative Names | eNOS ECNOS Constitutive NOS EC-NOS NOS3 NOSIII |
| Molecular Weight(MW) | 140 kDa |
| Tissue Specificity | This antibody detects endogenous level of total eNOS protein. |
| Entrez Gene | 4846 |
|---|---|
| Protein Accession | NP_000594.2 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using eNOS (Ab-1177).
Application
Western blot analysis of lysed extracts from Rat heart, Mouse heart and Rat kidney tissue using eNOS (Ab-1177).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
