Application
Western blot analysis of extracts from Jurkat cells and HepG2 cells using eNOS (Phospho-Thr495) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | eNOS (phospho Thr494) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cNOS, EC-NOS, ECNOS, NOS, NOS3 |
| Product description | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.1) Janssens S.P., J. Biol. Chem. 267:14519-14522(1992). |
| Immunogen | eNOS (phospho Thr494) antibody was raised against a peptide sequence around phosphorylation site of threonine 494 (R-K-K (p)-T-F derived from Human eNOS . |
| Modification | p-Thr494 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOS3 |
|---|---|
| Alternative Names | cNOS EC-NOS ECNOS NOS NOS3 |
| Molecular Weight(MW) | 140 kDa |
| Tissue Specificity | The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 494. |
| Entrez Gene | 4846 |
|---|---|
| Protein Accession | NP_000594.2 |
Application
Western blot analysis of extracts from Jurkat cells and HepG2 cells using eNOS (Phospho-Thr495) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
