Application
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ENTPD2 Antibody in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human brain lysate Lane 5: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.| Product Name | ENTPD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ectonucleoside triphosphate diphosphohydrolase 2, NTPDase 2, 361-, CD39 antigen-like 1, Ecto-ATP diphosphohydrolase 2, Ecto-ATPDase 2, Ecto-ATPase 2, ENTPD2, CD39L1 |
| Product description | ENTPD2 is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein.1) Chiang,W.C. Biochemistry 47 (33), 8775-8785 (2008) |
| Immunogen | This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENTPD2 |
|---|---|
| Alternative Names | Ectonucleoside triphosphate diphosphohydrolase 2 NTPDase 2 361- CD39 antigen-like 1 Ecto-ATP diphosphohydrolase 2 Ecto-ATPDase 2 Ecto-ATPase 2 ENTPD2 CD39L1 |
| Molecular Weight(MW) | 54 kDa |
Application
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ENTPD2 Antibody in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human brain lysate Lane 5: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
