Application
Immunohistochemistry of EPAC1 in rat brain tissue with EPAC1 antibody at 2.5 μg/mL.Application
Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 μg/mL.Application
Immunofluorescence of EPAC1 in rat brain tissue with EPAC1 antibody at 20 μg/mL.| Product Name | EPAC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EPAC1 Antibody: EPAC, EPAC1, bcm910, HSU79275, CAMP-GEFI, CGEF1, EPAC, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, EPAC 1 |
| Product description | EPAC1 Antibody: EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.1) de Rooij J, Zwartkruis FJ, Verheijen MH, et al. Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. Nature 1998; 396:474-7. |
| Immunogen | EPAC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EPAC1. The immunogen is located within amino acids 60 - 110 of EPAC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAPGEF3 |
|---|---|
| Alternative Names | EPAC1 Antibody: EPAC EPAC1 bcm910 HSU79275 CAMP-GEFI CGEF1 EPAC Rap guanine nucleotide exchange factor 3 Exchange factor directly activated by cAMP 1 EPAC 1 |
| Tissue Specificity | At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2. |
| Entrez Gene | 10411 |
|---|---|
| Protein Accession | NP_001092001 |
Application
Immunohistochemistry of EPAC1 in rat brain tissue with EPAC1 antibody at 2.5 μg/mL.
Application
Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 μg/mL.
Application
Immunofluorescence of EPAC1 in rat brain tissue with EPAC1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EPAC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EPAC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
