Application
Western blot analysis of extracts from HepG2 cells treated with PMA using EPB41 (Phospho-Tyr660/418) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | EPB41 (phospho Tyr660/418) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E41P, EPB4.1, band 4.1, P4.1 |
| Product description | Elliptocytosis is a hematologic disorder characterized by elliptically shaped erythrocytes and a variable degree of hemolytic anemia. Inherited as an autosomal dominant, elliptocytosis results from mutation in any one of several genes encoding proteins of the red cell membrane skeleton. The form discussed here is the one found in the 1950s to be linked to Rh blood group and more recently shown to be caused by a defect in protein 4.1. 'Rh-unlinked' forms of elliptocytosis are caused by mutation in the alpha-spectrin gene, the beta-spectrin gene, or the band 3 gene.1) Conboy J.G., Proc. Natl. Acad. Sci. U.S.A. 83:9512-9516(1986). |
| Immunogen | EPB41 (phospho Tyr660/418) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 660/418 (N-I-Y (p)-I-R)derived from Human EPB41. |
| Modification | p-Tyr660/418 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EPB41 |
|---|---|
| Alternative Names | E41P EPB4.1 band 4.1 P4.1 |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | The antibody detects endogenous levels of EPB41 only when phosphorylated at tyrosine 660/418. |
| Entrez Gene | 2035 |
|---|---|
| Protein Accession | NP_001159477.1 |
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using EPB41 (Phospho-Tyr660/418) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
