Application
Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | EPHA4 (phospho Tyr596) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EK8, hEK8, EPH-like kinase 8 |
| Product description | Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity.1) Rikova K., Cell 131:1190-1203(2007) [PubMed: 18083107]. |
| Immunogen | EPHA4 (phospho Tyr596) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 596 (R-T-Y (p)-V-D) derived from Human EPHA4 . |
| Modification | p-Tyr596 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EPHA4 |
|---|---|
| Alternative Names | EK8 hEK8 EPH-like kinase 8 |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | The antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596 |
| Entrez Gene | 2043 |
|---|---|
| Protein Accession | NP_004429.1 |
Application
Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
