Application
Western blot analysis in T47D cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-EphB1 Pab in mouse brain tissueApplication
Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d LApplication
Western blot analysis of lysates from A431, Hela, MDA-MB-453 cell line (from left to right), using EPHB1 Antibody (H970) at 1:1000 at each lane.| Product Name | EphB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ephrin type-B receptor 1, ELK, EPH tyrosine kinase 2, EPH-like kinase 6, EK6, hEK6, Neuronally-expressed EPH-related tyrosine kinase, NET, Tyrosine-protein kinase receptor EPH-2, EPHB1, ELK, EPHT2, HEK6, NET |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).1) Prevost, N., et al., Proc. Natl. Acad. Sci. U.S.A. 99(14):9219-9224 (2002). |
| Immunogen | This EphB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-984 amino acids from the C-terminal region of human EphB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | EPHB1 |
|---|---|
| Alternative Names | Ephrin type-B receptor 1 ELK EPH tyrosine kinase 2 EPH-like kinase 6 EK6 hEK6 Neuronally-expressed EPH-related tyrosine kinase NET Tyrosine-protein kinase receptor EPH-2 EPHB1 ELK EPHT2 HEK6 NET |
| Molecular Weight(MW) | 110 kDa |
Application
Western blot analysis in T47D cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-EphB1 Pab in mouse brain tissue
Application
Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d L
Application
Western blot analysis of lysates from A431, Hela, MDA-MB-453 cell line (from left to right), using EPHB1 Antibody (H970) at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
