Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of EphB4 using rabbit polyclonal EphB4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB4 gene (Lane 2).| Product Name | EphB4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ephrin type-B receptor 4, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11, EPHB4, HTK, MYK1, TYRO11 |
| Product description | Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.1) Steinle, J.J., et al., J. Biol. Chem. 277(46):43830-43835 (2002). Suenobu, S., et al., Biochem. Biophys. Res. Commun. 293(3):1124-1131 (2002). Wang, Z., et al., Blood 99(8):2740-2747 (2002). Wilson, M.D., et al., Nucleic Acids Res. 29(6):1352-1365 (2001). Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). |
| Immunogen | This EphB4 antibody is generated from rabbits immunized with human recombinant EphB4 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | EPHB4 |
|---|---|
| Alternative Names | Ephrin type-B receptor 4 Hepatoma transmembrane kinase Tyrosine-protein kinase TYRO11 EPHB4 HTK MYK1 TYRO11 |
| Molecular Weight(MW) | 108 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of EphB4 using rabbit polyclonal EphB4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB4 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
