Application
Immunohistochemistry staining of ERBB2 in breast carcinoma, neoplastic cells using ERBB2 Antibody.| Product Name | ERBB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1 |
| Product description | A tyrosine kinase receptor in the EGF-receptor family. ErbB2 does not bind ligand on its own, but acts as an amplifier in heterodimers with ErbB1, (EGFR), ErbB3, or ErbB4. Amplification of the ErbB2 gene and overexpression of ErbB2 protein is associated with breast cancer (~25% of cases), and anti-ErbB2 therapeutics (e.g. Herceptin, an anti-ErbB2 monoclonal antibody) are sometimes effective in treating these cases. Overexpression of ErbB2 has also been indicated as a factor in prostate cancer. |
| Immunogen | ERBB2 antibody was raised against a peptide located in the cytoplasmic domain of ERBB2 (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ERBB2 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:200, and immunofluorescence starting at 1:200 - 1:1000.: |
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| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERBB2 |
|---|---|
| Alternative Names | ERBB2 C-erb B2/neu protein CD340 HER2 Herstatin MLN 19 HER-2 Neu NGL Proto-oncogene c-ErbB-2 Proto-oncogene Neu CD340 antigen MLN19 p185erbB2 TKR1 |
| Tissue Specificity | BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Application
Immunohistochemistry staining of ERBB2 in breast carcinoma, neoplastic cells using ERBB2 Antibody.| Application Notes | ERBB2 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:200, and immunofluorescence starting at 1:200 - 1:1000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | ERBB2 antibody should be stored long term (months) at -80 ˚C and short term (days) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.1% sodium azide. |
