Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of 293 cells using ErbB3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of anti-ErbB3 Pab in mouse brain lysateApplication
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ERBB3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, ERBB3, HER3 |
| Product description | ErbB3, a member of the EGF receptor family, binds and is activated by neuregulins and NTAK. It potentially forms a heterodimer with each of the other ERBB receptors. This protein is predominantly expressed in epithelial tissues and brain. The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. Ligand-binding may increase phosphorylation on tyrosine residues and promote its association with the p85 subunit of phosphatidylinositol 3-kinase ErbB3 is overexpressed in a subset of human mammary tumors.1) Katoh, M., et al., Biochem. Biophys. Res. Commun. 192(3):1189-1197 (1993). Plowman, G.D., et al., Proc. Natl. Acad. Sci. U.S.A. 87(13):4905-4909 (1990). Kraus, M.H., et al., Proc. Natl. Acad. Sci. U.S.A. 86(23):9193-9197 (1989). |
| Immunogen | This ERBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human ERBB3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | ERBB3 |
|---|---|
| Alternative Names | Receptor tyrosine-protein kinase erbB-3 Proto-oncogene-like protein c-ErbB-3 Tyrosine kinase-type cell surface receptor HER3 ERBB3 HER3 |
| Molecular Weight(MW) | 148 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of 293 cells using ErbB3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of anti-ErbB3 Pab in mouse brain lysate
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
