v-ets erythroblastosis virus E26 oncogene like isoform 2 (ERG) is a transcriptional regulator. It may participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.1) Matsui, Y., et al., (2003) J. Biol. Chem. 278 (13), 11369-11375..
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ERG.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ERG antibody can be used for detection of ERG by ELISA at 1:62500. ERG antibody can be used for detection of ERG by western blot at 1.0&micr μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Jurkat 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
ERG antibody can be used for detection of ERG by ELISA at 1:62500. ERG antibody can be used for detection of ERG by western blot at 1.0&micr μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ERG antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt