Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.| Product Name | ESR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ESR1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1, ESTRR |
| Product description | Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.1) Giegling, I., (2008) Psychiatry Res 160 (1), 1-7. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ESR1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ESR1 antibody can be used for detection of ESR1 by ELISA at 1:62500. ESR1 antibody can be used for detection of ESR1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESR1 |
|---|---|
| Alternative Names | ESR1 DKFZp686N23123 ER ESR ESRA Era NR3A1 ESTRR |
| Molecular Weight(MW) | 66 kDa |
Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ESR1 antibody can be used for detection of ESR1 by ELISA at 1:62500. ESR1 antibody can be used for detection of ESR1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ESR1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
