Application
Immunofluorescence of ESRRB in human heart tissue with ESRRB antibody at 20 μg/mL.Application
Immunohistochemistry of ESRR8 in human heart tissue with ESRR8 antibody at 5 μg/mL.Application
Western blot analysis of ESRRB in human heart tissue lysate with ESRRB antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ESRRB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ESRRB Antibody: ERR2, ERRb, ESRL2, NR3B2, DFNB35, ERRB2, Steroid hormone receptor ERR2, ERR beta-2, ERR-beta |
| Product description | ESRRB Antibody: The estrogen-related receptor beta (ESRRB) was initially identified in mouse embryonic carcinoma stem cells and was found to be expressed exclusively in trophoblast progenitor cells between days 6.5 and 7.5 post coitum. Recent studies have shown that ESRRB is part of an POU5F1/Oct4-centered protein interaction network in embryonic stem (ES) cells. It is thought to interact with other ES transcription factors such as NANOG within this network, activating POU5F1 transcription and thereby sustaining self-renewal and pluripotency in ES cells.1) Pettersson K, Svensson K, Mattsson R, et al. Expression of a novel member of estrogen response element-binding nuclear receptors is restricted to the early stages of chorion formation during mouse embryogenesis. Mech. Dev. 1996; 54:211-23. |
| Immunogen | ESRRB antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ESRRB. The immunogen is located within amino acids 360 - 410 of ESRRB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ESRRB antibody can be used for detection of ESRRB by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESRRB |
|---|---|
| Alternative Names | ESRRB Antibody: ERR2 ERRb ESRL2 NR3B2 DFNB35 ERRB2 Steroid hormone receptor ERR2 ERR beta-2 ERR-beta |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunofluorescence of ESRRB in human heart tissue with ESRRB antibody at 20 μg/mL.
Application
Immunohistochemistry of ESRR8 in human heart tissue with ESRR8 antibody at 5 μg/mL.
Application
Western blot analysis of ESRRB in human heart tissue lysate with ESRRB antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | ESRRB antibody can be used for detection of ESRRB by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ESRRB antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ESRRB Antibody is supplied in PBS containing 0.02% sodium azide. |
