Application
Western blot analysis of lysed extracts from MCF-7, SK-OV-3 cells and Rat hippocampus tissue using Estrogen Receptor-α (Ab-104).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-104).| Product Name | Estrogen Receptor a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ER, ESR, Era, ESRA, ESTRR, NR3A1, CTNNB, OK/SW-cl.35, PRO2286, ER, ESR1, ESTR, ESTRA |
| Product description | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.1) Medunjanin S, et al. (2005) . J Biol Chem. 80 (38): 33006-33014. Dutertre M, et al. (2003) . Mol Endocrinol. 17 (7): 1296-1314. Chen D, et al. (2000) . Mol Cell. 6 (1): 127-137. Rogatsky I, et al. (1999) . J Biol Chem. 274 (32): 22296-22302. |
| Immunogen | Estrogen Receptor-a (Ab-104) antibody was raised against a peptide sequence around aa.102~106 (S-V-S-P-S) derived from Human Estrogen Receptor-α. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESR1 |
|---|---|
| Alternative Names | ER ESR Era ESRA ESTRR NR3A1 CTNNB OK/SW-cl.35 PRO2286 ER ESR1 ESTR ESTRA |
| Molecular Weight(MW) | 66 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Estrogen Receptor-α protein. |
| Entrez Gene | 2099 |
|---|---|
| Protein Accession | NP_000116.2 |
Application
Western blot analysis of lysed extracts from MCF-7, SK-OV-3 cells and Rat hippocampus tissue using Estrogen Receptor-α (Ab-104).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-104).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
