Application
Western blot analysis of lysed extracts from MCF cells using Estrogen Receptor-α (Ab-118).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-118). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Ab-118).| Product Name | Estrogen Receptor a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ER, ESR, Era, ESRA, ESTRR, NR3A1, HER2, MLN19, NEU, NGL, ER, ESR1, ESTR, ESTRA |
| Product description | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.1) H Yamaguchi, J Zhu, T Yu, et al. (2007) Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their differentiation state. Cell Biology International, 31 (6): 638-644. |
| Immunogen | Estrogen Receptor-a (Ab-118) antibody was raised against a peptide sequence around aa.116~120 (Q-L-S-P-F) derived from Human Estrogen Receptor-α. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESR1 |
|---|---|
| Alternative Names | ER ESR Era ESRA ESTRR NR3A1 HER2 MLN19 NEU NGL ER ESR1 ESTR ESTRA |
| Molecular Weight(MW) | 66 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Estrogen Receptor-α protein. |
| Entrez Gene | 2099 |
|---|---|
| Protein Accession | NP_000116.2 |
Application
Western blot analysis of lysed extracts from MCF cells using Estrogen Receptor-α (Ab-118).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-118). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Ab-118).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
