Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser118). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Phospho-Ser118).Application
Western blot analysis of lysed extracts from MDA cells untreated or treated with Estradiol using Estrogen Receptor-α (Phospho-Ser118).| Product Name | Estrogen Receptor a (phospho Ser118) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ER, ESR, Era, ESRA, ESTRR, NR3A1, BBC6, BCL2L8, ER, ESR1, ESTR, ESTRA |
| Product description | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.1) Ramsauer VP, et al. (2003) J Biol Chem; 278 (32): 30142-7. Jepson S, et al. (2002) Oncogene; 21 (49): 7524-32. Ren Z, et al. (2002) J Biol Chem; 277 (41): 38486-93. Eppenberger-Castori S, et al. (2001) J Clin Oncol; 19 (3): 645-56. |
| Immunogen | Estrogen Receptor-a (Phospho-Ser118) antibody was raised against a peptide sequence around phosphorylation site of serine 118 (Q-L-S (p) -P-F) derived from Human Estrogen Receptor-α. |
| Modification | p-Ser118 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESR1 |
|---|---|
| Alternative Names | ER ESR Era ESRA ESTRR NR3A1 BBC6 BCL2L8 ER ESR1 ESTR ESTRA |
| Molecular Weight(MW) | 66 kDa |
| Tissue Specificity | This antibody detects endogenous level of Estrogen Receptor-αonly when phosphorylated at serine 118. |
| Entrez Gene | 2099 |
|---|---|
| Protein Accession | NP_000116.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser118). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Phospho-Ser118).
Application
Western blot analysis of lysed extracts from MDA cells untreated or treated with Estradiol using Estrogen Receptor-α (Phospho-Ser118).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
