Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-alpha (Phospho-Tyr537) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | Estrogen Receptor-alpha (phospho Tyr537) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ER, ESR, ESR1, ESTR, NR3A1 |
| Product description | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.1) Sehime Temel, Endocrinology, Oct 2002; 143: 3974. |
| Immunogen | Estrogen Receptor-alpha (phospho Tyr537) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 537 (P-L-Y (p)-D-L) derived from Human Estrogen Receptor-alpha. |
| Modification | p-Tyr537 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESR1 |
|---|---|
| Alternative Names | ER ESR ESR1 ESTR NR3A1 |
| Molecular Weight(MW) | 66 kDa |
| Tissue Specificity | The antibody detects endogenous levels of ER-alpha only when phosphorylated at tyrosine 537. |
| Entrez Gene | 2099 |
|---|---|
| Protein Accession | NP_000116.2 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-alpha (Phospho-Tyr537) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
