Application
Immunofluorescence of ESX1 in human testis tissue with ESX1 antibody at 20 μg/mL.Application
Immunohistochemistry of ESX1 in human testis tissue with ESX1 antibody at 2.5 μg/mL.Application
Western blot analysis of ESX1 in human testis tissue lysate with ESX1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ESX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ESX1 Antibody: ESX1L, ESXR1, ESX1L, ESX1R, Homeobox protein ESX1, Extraembryonic, spermatogenesis, homeobox 1 |
| Product description | ESX1 Antibody: Homeobox proteins are transcription factors that contain a helix-turn-helix DNA binding domain termed the homeodomain. ESX1 is an X-linked homeobox gene primarily expressed in the placenta and testis and contains two functional domains: the homeodomain and the proline-rich domain. During embryogenesis, ESX1 is expressed in the extraembryonic tissues, including the endoderm of the visceral yolk sac, the ectoderm of the chorion and the labyrinthine trophoblast of the chorioallantoic placenta. ESX1 can act like a transcriptional repressor to the human oncogene K-ras and treatment of human cancer cells with an ESX1 protein fragment containing the homeodomain reduces the tumorgenicity of cells containing oncogenic K-ras mutations, suggesting ESX1 may be useful as a therapeutic treatment for these cancers.1) Gehring WJ, Affolter M and Burglin T. Homeodomain proteins. Annu. Rev. Biochem.1994; 63:487-526. |
| Immunogen | ESX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human ESX1. The immunogen is located within amino acids 140 - 190 of ESX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ESX1 antibody can be used for detection of ESX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESX1 |
|---|---|
| Alternative Names | ESX1 Antibody: ESX1L ESXR1 ESX1L ESX1R Homeobox protein ESX1 Extraembryonic spermatogenesis homeobox 1 |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
Application
Immunofluorescence of ESX1 in human testis tissue with ESX1 antibody at 20 μg/mL.
Application
Immunohistochemistry of ESX1 in human testis tissue with ESX1 antibody at 2.5 μg/mL.
Application
Western blot analysis of ESX1 in human testis tissue lysate with ESX1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | ESX1 antibody can be used for detection of ESX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ESX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ESX1 Antibody is supplied in PBS containing 0.02% sodium azide. |
