Application
Antibody used in WB on Human Hela at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.Application
Antibody used in WB on Human HepG2 at 1 ug/ml.| Product Name | ETFA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ETFA, EMA, GA2, MADD |
| Product description | ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.1) Chiong, M.A., Mol. Genet. Metab. 92 (1-2), 109-114 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ETFA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ETFA antibody can be used for detection of ETFA by ELISA at 1:62500. ETFA antibody can be used for detection of ETFA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ETFA |
|---|---|
| Alternative Names | ETFA EMA GA2 MADD |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in WB on Human Hela at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | ETFA antibody can be used for detection of ETFA by ELISA at 1:62500. ETFA antibody can be used for detection of ETFA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ETFA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
