Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody.Application
Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | ETS1 (phospho Thr38) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p54, ETS1, ETS-1, C-ets-1 protein |
| Product description | Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.1) Tsukasa Higuchi, Mol. Cell. Biol., May 2007; 27: 3353 - 3366. |
| Immunogen | ETS1 (phospho Thr38) antibody was raised against a peptide sequence around phosphorylation site of threonine 38 (L-L-T (p)-P-S) derived from Human ETS1 . |
| Modification | p-Thr38 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ETS1 |
|---|---|
| Alternative Names | p54 ETS1 ETS-1 C-ets-1 protein |
| Molecular Weight(MW) | 54 kDa |
| Tissue Specificity | The antibody detects endogenous levels of ETS1 only when phosphorylated at threonine 38. |
| Entrez Gene | 2113 |
|---|---|
| Protein Accession | NP_035938.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody.
Application
Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
