Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of ETS1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | ETS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein C-ets-1, p54, ETS1, EWSR2 |
| Product description | ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (summary by Dwyer et al., 2007 [PubMed 17986575]).1) Harris, T.A., et al. Arterioscler. Thromb. Vasc. Biol. 30(10):1990-1997(2010) He, C.F., et al. Lupus 19(10):1181-1186(2010) Russell, L., et al. Cytokine 51(3):217-226(2010) Li, T., et al. Pharm Biol 48(2):161-165(2010) Kunderfranco, P., et al. PLoS ONE(5), E10547 (2010) : |
| Immunogen | This ETS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the N-terminal region of human ETS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ETS1 |
|---|---|
| Alternative Names | Protein C-ets-1 p54 ETS1 EWSR2 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of ETS1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
