EVX1 is a member of the vertebrate eve-related homeo box family. This protein acts as a transcriptional repressor. A similar protein in mice is critical for early embryogenesis. This gene encodes a member of the even-skipped homeobox family characterized by the presence of a homeodomain closely related to the Drosophila even-skipped (eve) segmentation gene of the pair-rule class. The encoded protein may play an important role as a transcriptional repressor during embryogenesis.1) Briata, P., (1995) J. Biol. Chem. 270 (46), 27695-27701 .
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EVX1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
EVX1 antibody can be used for detection of EVX1 by ELISA at 1:62500. EVX1 antibody can be used for detection of EVX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
EVX1 antibody can be used for detection of EVX1 by ELISA at 1:62500. EVX1 antibody can be used for detection of EVX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EVX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.