Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using EWS Antibody.| Product Name | EWS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EWS, bK984G1.4, EWSR1 |
| Product description | This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.1) Ubhi T. B. S., Rainey D. R., Craig A. R., Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases |
| Immunogen | EWS antibody was raised against a peptide sequence around aa.213~217 (T-Y-G-Q-P) derived from Human EWS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EWSR1 |
|---|---|
| Alternative Names | EWS bK984G1.4 EWSR1 |
| Molecular Weight(MW) | 85 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total EWS protein. |
| Entrez Gene | 2130 |
|---|---|
| Protein Accession | NP_053733.2 |
Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using EWS Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
