Application
Immunocytochemistry of EZH2 in 293 cells with EZH2 antibody at 10 μg/mL.Application
Immunofluorescence of EZH2 in 293 cells with EZH2 antibody at 20 μg/mL.Application
Western blot analysis of EZH2 in 293 cell lysate with EZH2 antibody at 1 μg/mL.| Product Name | EZH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EZH2 Antibody: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A, Histone-lysine N-methyltransferase EZH2 |
| Product description | EZH2 Antibody: EZH2 was initially identified as a homolog of the drosophila Enhancer of Zeste through exon trap screening of chromosome 21. Both EZH2 and the related protein EZH1 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair. Overexpression of EZH2 has been reported as a marker for advanced and metastatic cancers.1) Chen H, Rossier C, and Antonarakis SE. Cloning of a human homolog of the Drosophila enhancer of zeste gene (EZH2) that maps to chromosome 21q22.2. Genomics 1996; 38:30-7 |
| Immunogen | EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2. The immunogen is located within amino acids 190 - 240 of EZH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| EZH2 antibody can be used for detection of EZH2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EZH2 |
|---|---|
| Alternative Names | EZH2 Antibody: WVS ENX1 EZH1 KMT6 WVS2 ENX-1 EZH2b KMT6A Histone-lysine N-methyltransferase EZH2 |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1. |
Application
Immunocytochemistry of EZH2 in 293 cells with EZH2 antibody at 10 μg/mL.
Application
Immunofluorescence of EZH2 in 293 cells with EZH2 antibody at 20 μg/mL.
Application
Western blot analysis of EZH2 in 293 cell lysate with EZH2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | EZH2 antibody can be used for detection of EZH2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EZH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EZH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
