Application
Western blot analysis of extracts from 293 cells treated with UV using Phospho-Ezrin (Tyr146) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Ezrin (phospho Tyr146) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytovillin, EZRI, p81, VIL2, Villin 2 |
| Product description | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.1) Murchie R, et al. (2014)Proc Natl Acad Sci U S A 111, 693-8. Kawahara T, et al. (2013)PLoS One 8, e79654 . Wang Y, Lazo JS (2012)Oncogene 31, 818-27. |
| Immunogen | Ezrin (phospho Tyr146) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 146 (S-G-Y (p)-L-S) derived from Human Ezrin. |
| Modification | p-Tyr146 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EZR |
|---|---|
| Alternative Names | Cytovillin EZRI p81 VIL2 Villin 2 |
| Molecular Weight(MW) | 69 kDa |
| Tissue Specificity | The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 146. |
| Entrez Gene | 7430 |
|---|---|
| Protein Accession | NP_001104547.1 |
Application
Western blot analysis of extracts from 293 cells treated with UV using Phospho-Ezrin (Tyr146) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
