Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Ezrin (Phospho-Tyr353).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353).| Product Name | Ezrin (phospho Tyr353) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CVL, CVIL, VIL2, HEL-S-105, ESR, NR3A1, Cytovillin, EZRI, Villin 2, p81 |
| Product description | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.1) Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490. Wang Q, et al. (2003) J Biol Chem 278 (48): 47731-47743. Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96 (13): 7300-7305. Crepaldi T, et al. (1997) J Cell Biol 138 (2): 423-34. |
| Immunogen | Ezrin (Phospho-Tyr353) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y (p) -E-E) derived from Human EZRIN. |
| Modification | p-Tyr353 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EZR |
|---|---|
| Alternative Names | CVL CVIL VIL2 HEL-S-105 ESR NR3A1 Cytovillin EZRI Villin 2 p81 |
| Molecular Weight(MW) | 81 kDa |
| Tissue Specificity | This antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353. |
| Entrez Gene | 7430 |
|---|---|
| Protein Accession | NP_001104547.1 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Ezrin (Phospho-Tyr353).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
