Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life.Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Rossi, E., (2008) Thromb. Haemost. 99 (6), 1030-1034.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human F2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
F2 antibody can be used for detection of F2 by ELISA at 1:312500. F2 antibody can be used for detection of F2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Placenta at 5 ug/ml.
Application
Antibody used in IHC on Human Placenta at 5 ug/ml.
Application
Antibody used in WB on Human 721_B at 1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
F2 antibody can be used for detection of F2 by ELISA at 1:312500. F2 antibody can be used for detection of F2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store F2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.