Application
Antibody used in WB on Transfected 293T at 5.0 ug/ml.Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.| Product Name | FARS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FARS2, FARS1, HSPC320, PheRS, dJ520B18.2, COXPD14 |
| Product description | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS2 is a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm.Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS1 encodes a mitochondrially-located phenylalanine-tRNA synthetase (PheRS) which consists of a single polypeptide chain unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and the eukaryotic cytoplasm.1) Brandenberger, R., (2004) Nat. Biotechnol. 22 (6), 707-716. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FARS2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| FARS2 antibody can be used for detection of FARS2 by ELISA at 1:62500. FARS2 antibody can be used for detection of FARS2 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FARS2 |
|---|---|
| Alternative Names | FARS2 FARS1 HSPC320 PheRS dJ520B18.2 COXPD14 |
| Molecular Weight(MW) | 50 kDa |
Application
Antibody used in WB on Transfected 293T at 5.0 ug/ml.
Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | FARS2 antibody can be used for detection of FARS2 by ELISA at 1:62500. FARS2 antibody can be used for detection of FARS2 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FARS2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
