Application
Western blot analysis of FDFT1 using rabbit polyclonal FDFT1 Antibody (A155) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FDFT1 gene.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with FDFT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-FDFT1 Antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2417b) in A375 cell line lysate.| Product Name | FDFT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FDFT1 catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.1) Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). |
| Immunogen | This FDFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-170 amino acids from the Central region of human FDFT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | FDFT1 |
|---|---|
| Alternative Names | Squalene synthase SQS SS FPP:FPP farnesyltransferase Farnesyl-diphosphate farnesyltransferase FDFT1 |
| Molecular Weight(MW) | 48 kDa |
Application
Western blot analysis of FDFT1 using rabbit polyclonal FDFT1 Antibody (A155) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FDFT1 gene.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with FDFT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-FDFT1 Antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2417b) in A375 cell line lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
