Application
Immunocytochemistry of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.Application
Immunofluorescence of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.Application
Western blot analysis of FGF4 in NIH 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | FGF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FGF4 Antibody: Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.1) Powers CJ, McLeskey SW, and Wellstein A. Fibroblast growth factors, their receptors and signaling. Endocr. Relat. Cancer2000; 7:165-97. |
| Immunogen | FGF4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4. The immunogen is located within the last 50 amino acids of FGF4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FGF4 |
|---|---|
| Alternative Names | FGF4 Antibody: HST KFGF HST-1 HSTF1 K-FGF HBGF-4 HST KS3 Fibroblast growth factor 4 Heparin secretory-transforming protein 1 FGF-4 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
Application
Immunocytochemistry of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.
Application
Immunofluorescence of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.
Application
Western blot analysis of FGF4 in NIH 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FGF4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FGF4 Antibody is supplied in PBS containing 0.02% sodium azide. |
