Application
Immunohistochemistry of FGFR3 in rat brain tissue with FGFR3 antibody at 2.5 μg/mL.Application
Western blot analysis of FGFR3 in SK-N-SH cell lysate with FGFR3 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of FGFR3 in rat brain tissue with FGFR3 antibody at 20 μg/mL.| Product Name | FGFR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FGFR3 Antibody: FGFR3 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis (1). It is required for normal skeleton development and promotes apoptosis in chondrocytes and cancer cell proliferation. FGFR3 protein contains three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain (1,2). It is alternatively spliced to produce three isoforms that are expressed in brain, kidney and testis. Defects in FGFR3 are associated with several diseases, including achondroplasia and hypochondroplasia (3,4). Mutations in FGFR3 are also a cause of some bladder and cervical cancers (5).1) Keegan K, Johnson DE, and Williams LT. Isolation of an additional member of the fibroblast growth factor receptor family, FGFR-3. Proc. Natl. Acad. Sci. USA 1991; 88:1095-9. |
| Immunogen | FGFR3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FGFR3. The immunogen is located within amino acids 630 - 680 of FGFR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FGF3 antibody can be used for detection of FGFR3 by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FGFR3 |
|---|---|
| Alternative Names | FGFR3 Antibody: ACH CEK2 JTK4 CD333 HSFGFR3EX Fibroblast growth factor receptor 3 FGFR-3 |
| Molecular Weight(MW) | Predicted: 89 kDa Observed: 9+2, 98 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (95%) |
| Tissue Specificity | FGFR3 antibody is human, mouse and rat reactive. At least three isoforms of FGFR3 are known to exist; this antibody will detect all three. |
| Entrez Gene | 2261 |
|---|---|
| Protein Accession | NP_001156685 |
Application
Immunohistochemistry of FGFR3 in rat brain tissue with FGFR3 antibody at 2.5 μg/mL.
Application
Western blot analysis of FGFR3 in SK-N-SH cell lysate with FGFR3 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of FGFR3 in rat brain tissue with FGFR3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | FGF3 antibody can be used for detection of FGFR3 by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FGFR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | FGFR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
