Application
Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.Application
Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.Application
Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | Fibulin 3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fibulin 3 Antibody: DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, FBLN3, FIBL-3, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5 |
| Product description | Fibulin 3 Antibody: Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target.1) Kobayashi N, Kostka G, Garbe JH, et al. A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization. J. Biol. Chem. 2007; 282:11805-16. |
| Immunogen | Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3. The immunogen is located within amino acids 410 - 460 of Fibulin 3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFEMP1 |
|---|---|
| Alternative Names | Fibulin 3 Antibody: DHRD DRAD FBNL MLVT MTLV S1-5 FBLN3 FIBL-3 EGF-containing fibulin-like extracellular matrix protein 1 Extracellular protein S1-5 |
| Molecular Weight(MW) | Predicted: 52 kDa Observed: 47 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins. |
| Entrez Gene | 2202 |
|---|---|
| Protein Accession | NP_001034437 |
Application
Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.
Application
Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.
Application
Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Fibulin 3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Fibulin 3 Antibody is supplied in PBS containing 0.02% sodium azide. |
