Application
Western blot analysis of FKBP4 using rabbit polyclonal FKBP4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FKBP4 gene.Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of FKBP4 Antibody in Hela,MCF7,T47D cell line lysates(35ug/lane).| Product Name | FKBP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FKBP4 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy.1) Tatro,E.T.,Neuroimmune Pharmacol(2), 218-226 (2009) |
| Immunogen | This FKBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the Central region of human FKBP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | FKBP4 |
|---|---|
| Alternative Names | Peptidyl-prolyl cis-trans isomerase FKBP4 PPIase FKBP4 51 kDa FK506-binding protein FKBP51 52 kDa FK506-binding protein 52 kDa FKBP FKBP-52 59 kDa immunophilin p59 FK506-binding protein 4 FKBP-4 FKBP59 HSP-binding immunophilin HBI Immunophili |
| Molecular Weight(MW) | 52 kDa |
Application
Western blot analysis of FKBP4 using rabbit polyclonal FKBP4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FKBP4 gene.
Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of FKBP4 Antibody in Hela,MCF7,T47D cell line lysates(35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
