Application
Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.Application
Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.Application
Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/mL.| Product Name | FLASH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLASH Antibody: CED-4, FLASH, RIP25, KIAA1315, CASP8-associated protein 2, FLICE-associated huge protein |
| Product description | FLASH Antibody: A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.1) Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85 |
| Immunogen | FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP8AP2 |
|---|---|
| Alternative Names | FLASH Antibody: CED-4 FLASH RIP25 KIAA1315 CASP8-associated protein 2 FLICE-associated huge protein |
| Molecular Weight(MW) | 220 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.
Application
Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.
Application
Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FLASH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FLASH Antibody is supplied in PBS containing 0.02% sodium azide. |
