Application
Immunofluorescence of FLI1 in HeLa cells with MUC1 antibody at 5 μg/mL.| Product Name | FLI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLI1 Antibody : ATOD2, Filaggrin |
| Product description | The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1). FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3). The FLI1 gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4). Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5).1) Hollenhorst PC, Jones DA, and Graves BJ. Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors. Nuc. Acids Res. 2004; 32:5693-702. |
| Immunogen | FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1. The immunogen is located within the last 50 amino acids of FLI1 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| FLI1 antibody can be used for detection of FLI1 by Western blot at 0.5 - 1 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLI1 |
|---|---|
| Alternative Names | FLI1 Antibody : ATOD2 Filaggrin |
| Molecular Weight(MW) | Predicted: 50 kDa Observed: 52kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist; this antibody will detect all four. |
Application
Immunofluorescence of FLI1 in HeLa cells with MUC1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | FLI1 antibody can be used for detection of FLI1 by Western blot at 0.5 - 1 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FLI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | FLI1 Antibody is supplied in PBS containing 0.02% sodium azide. |
