Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of FLNA Antibody (Y1046) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
FLNA Antibody (Y1046) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FLNA Antibody (Y1046) for immunohistochemistry.Application
Western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the FLNA antibody detected the FLNA protein (arrow).| Product Name | FLNA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Actin-binding protein 280, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.1) Ravid,D., Exp. Cell Res. 314 (15), 2762-2773 (2008) |
| Immunogen | This FLNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1025-1054 amino acids from human FLNA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLNA |
|---|---|
| Alternative Names | Filamin-A FLN-A Actin-binding protein 280 ABP-280 Alpha-filamin Endothelial actin-binding protein Filamin-1 Non-muscle filamin FLNA FLN FLN1 |
| Molecular Weight(MW) | 281 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of FLNA Antibody (Y1046) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
FLNA Antibody (Y1046) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FLNA Antibody (Y1046) for immunohistochemistry.
Application
Western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the FLNA antibody detected the FLNA protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
